Fluo-3, AM是最常用的檢測細(xì)胞內(nèi)鈣離子濃度的熒光探針之一。它穿透細(xì)胞膜進(jìn)入細(xì)胞后被細(xì)胞內(nèi)的酯酶剪切形成Fluo-3 ，從而被滯留在細(xì)胞內(nèi)，F(xiàn)luo-3 若以游離配體形式存在時(shí)幾乎是非熒光性的，但是當(dāng)它與細(xì)胞內(nèi)鈣離子結(jié)合后可以產(chǎn)生較強(qiáng)的熒光，最大激發(fā)波長為506nm ，最大發(fā)射波長為526nm 。實(shí)際檢測時(shí)推薦使用的激發(fā)波長為488nm 左右，發(fā)射波長為 525～530nm ?？梢允褂眉す夤簿劢癸@微鏡或流式細(xì)胞儀檢測細(xì)胞內(nèi)鈣離子濃度的變化。

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標(biāo)題:Lung protection of Chimonanthus nitens Oliv. essential oil driven by the control of intestinal disorders and dysbiosis through gut-lung crosstalk

成員:Teng Li, Min Wan, Cheng Qing, Xiuping Guan, Jinchan Pi, Hao Lv, Wenjuan Li

論文因子:6.1 發(fā)表期刊:LIFE SCIENCES pmid:37805165

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標(biāo)題:Self-promoted electroactive biomimetic mineralized scaffolds for bacteria-infected bone regeneration

成員:Li Zixin, He Danqing, Guo Bowen, Wang Zekun, Yu Huajie, Wang Yu, Jin Shanshan, Yu Min, Zhu Lisha, Chen Liyuan, Ding Chengye, Wu Xiaolan, Wu Tianhao, Gong Shiqiang, Mao Jing, Zhou Yanheng, Luo Dan, Liu Yan

論文因子:16.6 發(fā)表期刊:Nature Communications pmid:37907455

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標(biāo)題:GRPR down-regulation inhibits spermatogenesis through Ca2+ mediated by PLC汕/IP3R signaling pathway in long-term formaldehyde-exposed rats

成員:Yan-qi Yang, Rui-fang Sun, Pan Ge, Wen-Xing Li, Xiang Zhang, Jian Zhang, Lu Ye, Nan Zhang, Si-yu Wang, Mo-qi Lv, Dang-xia Zhou

論文因子:4.3 發(fā)表期刊:FOOD AND CHEMICAL TOXICOLOGY pmid:37604300

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標(biāo)題:A microfluidic array enabling generation of identical biochemical stimulating signals to trapped biological cells for single-cell dynamics

成員:Miao Yu, Yong-Jiang Li, Yu-Nong Yang, Chun-Dong Xue, Gui-Yang Xin, Bo Liu, Kai-Rong Qin

論文因子:6.1 發(fā)表期刊:TALANTA pmid:37699267

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標(biāo)題:Epigenetically upregulated oncoprotein PLCE1 drives esophageal carcinoma angiogenesis and proliferation via activating the PI-PLC -NF- B signaling pathway and VEGF-C/ Bcl-2 expression

成員:Yunzhao Chen, Dandan Wang, Hao Peng, Xi Chen, Xuep

論文因子:7.776 發(fā)表期刊:Molecular Cancer pmid:30609930

注意：以上文獻(xiàn) 僅供參考

鈣熒光探針Fluo-3，AM(5mM 無水DMSO溶液) 121714-22-5

鈣熒光探針Fluo-3，AM(5mM 無水DMSO溶液) 121714-22-5